Apg3 Recombinant Rabbit Monoclonal Antibody [JU00-35]

About this product

Atg3 (autophagy-related protein 3), also known as APG3-like, hAPG3 or PC3-96, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. Atg3 catalyzes the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, a reaction that is essential for autophagy (a cellular process that allows for the degradation of organelles and bulk cellular proteins). The process of forming the Atg8-PE conjugate begins with the removal of the C-terminal arginine residue of Atg8 by Atg4, a cysteine protease. The, now exposed, glycine residue is then activated by Atg7 and is then transferred to Atg3 for the final conjugation to PE. This last step can be accelerated by the presence of the Atg12-Atg5 conjugate which functions similarly to an E3 enzyme.

Specifications

Catalog number

ET1706-29

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

2610016C12Rik antibody Apg 3 antibody APG3 antibody APG3 autophagy 3 like antibody APG3 like antibody APG3, S. cerevisiae, homolog of antibody APG3-like antibody APG3L antibody Apg3p antibody ATG 3 antibody ATG3 antibody ATG3 autophagy related 3 homolog antibody ATG3 autophagy related 3 homolog (S. cerevisiae) antibody ATG3_HUMAN antibody Autophagy 3, S. cerevisiae, homolog of antibody Autophagy Apg3p/Aut1p like antibody autophagy related 3 antibody Autophagy related protein 3 antibody Autophagy-related protein 3 antibody DKFZp564M1178 antibody FLJ22125 antibody hApg3 antibody MGC15201 antibody OTTHUMP00000214547 antibody OTTHUMP00000214548 antibody PC3 96 antibody Protein PC3-96 antibody Ubiquitin-like-conjugating enzyme ATG3 antibody

Abbreviation

APG3-like, hApg3, ATG3, APG3, APG3L

Swiss Prot

Q9NT62 Human, Q9CPX6 Mouse, Q6AZ50 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Apg3 1-50 / 314.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

K562 cell lysate, HL-60 cell lysate, Hela cell lysate, Jurkat cell lysate, human tonsil tissue, human thyroid gland tissue, human colon carcinoma tissue, mouse small intestine tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

40 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes