Mad2L2 Recombinant Rabbit Monoclonal Antibody [JU99-23]

About this product

Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T-47D, was shown to complete mitosis in the presence of Nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during pro-metaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with Insulin receptor (IR), but not IGF-IR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and, if the process is not complete, MAD2B delays the onset of anaphase.

Specifications

Catalog number

ET1706-38

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Homolog of REV7 S cerevisiae antibody hREV7 antibody MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody MAD2 homolog antibody MAD2 like 2 antibody MAD2 mitotic arrest deficient like 2 antibody MAD2-like protein 2 antibody MAD2B antibody Mad2l2 antibody MD2L2_HUMAN antibody Mitotic Arrest Deficient 2 L2 antibody Mitotic arrest deficient 2-like protein 2 antibody Mitotic arrest deficient homolog like 2 antibody Mitotic arrest deficient like 2 (yeast) antibody Mitotic arrest deficient yeast homolog antibody Mitotic spindle assembly checkpoint protein MAD2B antibody Polymerase (DNA directed) zeta 2 accessory subunit antibody POLZ2 antibody REV 7 antibody REV7 antibody REV7 homolog antibody Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody

Abbreviation

MAD2-like protein 2, hREV7, MAD2L2, MAD2B, REV7

Swiss Prot

Q9UI95 Human, Q9D752 Mouse, D3Z8D9 Rat

Cellular Locus

Nucleus. Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Mad2L2 aa 162-211 / 211.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IP

Positive Control

SiHa cell lysates, K562 cell lysates, rat lung tissue, mouse colon tissue, human tonsil tissue, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: 1:10-1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

24 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes