ZAP70 Recombinant Rabbit Monoclonal Antibody [JU08-39]

About this product

The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and e chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short 5 amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with z and undergoes tyrosine phosphorylation following TCR stimulation.

Specifications

Catalog number

ET1706-42

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

70 kDa zeta associated protein antibody 70 kDa zeta-associated protein antibody EC 2.7.10.2 antibody FLJ17670 antibody FLJ17679 antibody Selective T cell defect antibody SRK antibody STD antibody Syk related tyrosine kinase antibody Syk-related tyrosine kinase antibody Truncated ZAP kinase antibody Tyrosine protein kinase ZAP70 antibody Tyrosine-protein kinase ZAP-70 antibody TZK antibody ZAP 70 antibody ZAP70 antibody ZAP70_HUMAN antibody Zeta chain associated protein kinase 70kD antibody Zeta chain associated protein kinase 70kDa antibody Zeta chain associated protein kinase 70kDa isoform 1 antibody Zeta chain associated protein kinase 70kDa isoform 2 antibody Zeta chain of T cell receptor associated protein kinase 70 antibody Zeta chain TCR associated protein kinase 70kD antibody Zeta chain TCR associated protein kinase 70kDa antibody

Abbreviation

ZAP70, SRK

Swiss Prot

P43403 Human

Cellular Locus

Cytoplasm. Cell membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human ZAP70 aa 570-619 / 619.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, IP

Positive Control

Human thymus tissue lysates, Jurkat cell lysates, LOVO, SH-SY-5Y, human tonsil tissue, human spleen tissue, 商品名 was done on Jurkat.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: 1:10-1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 70 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes