ADAR Recombinant Rabbit Monoclonal Antibody [JU99-33]

About this product

RNA-specific adenosine deaminase (ADAR1, DSH, IFI4, p136, DRADA, DSRAD, K88dsRBP) mediates RNA editing by destabilizing double stranded RNA through deamination of adenosine to inosine in structured or double-stranded RNAs. ADAR1 is expressed from an interferon-response promoter and has a Z-DNA/Z-RNA binding domain at its N-terminus. ADAR1 co-localizes with SUMO-1 in a subnucleolar region that is distinct from the fibrillar center, the dense fibrillar component and the granular component. Localization of nuclear ADAR1 is under the influence of a nucleolar localization signal (NoLS) in the middle of ADAR1 and the exporting activity of the nuclear exporter signal (NES) near the N terminus. ADAR1 upregulates nuclear factor 90 (NF90)-mediated gene expression by interacting with NF110, NF90 and NF45. ADAR1 binds short interfering RNA (siRNA), and gene silencing by siRNA is significantly more effective in mouse fibroblasts homozygous for an ADAR1 null mutation than in wild-type cells. ADAR1 may limit the efficacy of siRNA in mammalian cells.

Specifications

Catalog number

ET7106-72

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

136 kDa double-stranded RNA-binding protein antibody 136kDa double stranded RNA binding protein antibody Adar 1 antibody ADAR antibody Adar1 antibody Adenosine deaminase acting on RNA 1 A antibody Adenosine deaminase RNA specific 1 antibody Adenosine deaminase RNA specific antibody Adenosine deaminase that act on RNA antibody AGS6 antibody AV242451 antibody Double stranded RNA specific adenosine deaminase antibody Double-stranded RNA-specific adenosine deaminase antibody Double-stranded RNA-specific editase Adar antibody DRADA antibody Dsh antibody Dsrad antibody DSRAD_HUMAN antibody dsRNA adenosine deaminase antibody EC 3.5.4.- antibody G1P1 antibody IFI 4 antibody IFI-4 antibody IFI4 antibody Ifi4 protein antibody Interferon induced protein 4 antibody Interferon inducible protein 4 antibody Interferon-inducible protein 4 antibody K88DSRBP antibody mZaADAR antibody P136 antibody Pre-mRNA adenosine deaminase antibody RNA adenosine deaminase 1 antibody RNA-editing deaminase 1 antibody RNA-editing enzyme 1 antibody

Abbreviation

DRADA, p136, IFI-4, ADAR, ADAR1, DSRAD, G1P1, IFI4

Swiss Prot

P55265 Human

Cellular Locus

Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human ADAR aa 180-280 / 1,226.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IF-Tissue, FC

Positive Control

HeLa cell lysate, 293T cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, HeLa, human colon cancer tissue, human kidney tissue, human lung cancer tissue, human pancreas tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:200-1:1,000 ;IF-Tissue: 1:50 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 136 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes