NR1D1 Recombinant Rabbit Monoclonal Antibody [JU34-31]

About this product

Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARTNL/BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nucleotides (RevDR-2).

Specifications

Catalog number

ET7106-92

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

EAR-1 antibody EAR1 antibody ERBA-related 1 antibody hRev antibody Nr1d1 antibody NR1D1_HUMAN antibody Nuclear receptor Rev ErbA alpha antibody Nuclear receptor subfamily 1 group D member 1 antibody Rev erbAalpha antibody Rev erbalpha antibody Rev-erbA-alpha antibody Rev-ErbAalpha antibody Reverba antibody THRA1 antibody THRAL antibody Thyroid hormone receptor, alpha like antibody Thyroid hormone receptor, alpha-1- like antibody V-erbA-related protein 1 antibody

Abbreviation

EAR-1, NR1D1, EAR1, HREV, THRAL

Swiss Prot

P20393 Human, Q3UV55 Mouse, Q63503 Rat

Cellular Locus

Nucleus, Cytoplasm, Cell projection, dendrite, dendritic spine.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human NR1D1 aa 510-614 / 614.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

HepG2 cell lysate, SiHa cell lysate, human colon cancer tissue, mouse fallopian tube tissue, mouse kidney tissue, mouse brain tissue, rat brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 67 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes