SFPQ Recombinant Rabbit Monoclonal Antibody [JU33-99]

About this product

DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation.

Specifications

Catalog number

ET7107-02

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

100 kDa DNA pairing protein antibody 100 kDa DNA-pairing protein antibody 100 kDa subunit antibody DNA binding p52/p100 complex 100 kDa subunit antibody DNA-binding p52/p100 complex antibody hPOMp100 antibody Polypyrimidine tract binding protein associated splicing factor antibody Polypyrimidine tract-binding protein-associated-splicing factor antibody POMP100 antibody PPP1R140 antibody proline- and glutamine-rich antibody Protein phosphatase 1 regulatory subunit 140 antibody PSF antibody PTB associated splicing factor antibody PTB-associated-splicing factor antibody Sfpq antibody SFPQ_HUMAN antibody Splicing factor antibody Splicing factor proline and glutamine rich antibody Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody Splicing factor proline/glutamine rich antibody

Abbreviation

hPOMp100, PSF, PTB-associated-splicing factor, SFPQ

Swiss Prot

P23246 Human, Q8VIJ6 Mouse,

Cellular Locus

Nucleus. Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human SFPQ aa 658-707 / 707.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, IF-Tissue

Positive Control

Jurkat cell lysate, 293T cell lysate, HeLa cell lysate, K-562 cell lysate, Daudi cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse testis tissue lysate, Mouse lung tissue lysate, mouse brain tissue, rat brain tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:10-1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 76 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes