GRASP65 Recombinant Rabbit Monoclonal Antibody [JB36-34]

About this product

Golgi reassembly-stacking protein of 65 kDa (GRASP65) also known as Golgi reassembly-stacking protein 1 (GORASP1) is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The GRASP65 protein is a peripheral membrane protein anchored to the lipid bilayer through myristoylation of a glycine residue near the protein's amino terminus. It is involved in establishing the stacked structure of the Golgi apparatus and linking the stacks into larger ribbons in vertebrate cells. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. GRASP65 can form a complex with the Golgi matrix protein GM130, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined.

Specifications

Catalog number

ET7107-13

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

FLJ23443 antibody Golgi peripheral membrane protein p65 antibody Golgi phosphoprotein 5 antibody Golgi reassembly and stacking protein 1 antibody Golgi reassembly and stacking protein 65 kDa antibody Golgi reassembly and stacking protein, 65-kD antibody Golgi reassembly stacking protein 1 65kDa antibody Golgi reassembly stacking protein 1 antibody Golgi reassembly stacking protein of 65 kDa antibody Golgi reassembly-stacking protein 1 antibody Golgi reassembly-stacking protein of 65 kDa antibody GOLPH 5 antibody GOLPH5 antibody Gorasp 1 antibody GORASP1 antibody GORS1_HUMAN antibody GRASP 65 antibody GRASP65 antibody MGC118894 antibody MGC118897 antibody P65 antibody

Abbreviation

GOLPH5, GRASP65, GORASP1, GOLPH5, GRASP65

Swiss Prot

Q9BQQ3 Human

Cellular Locus

Golgi apparatus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human GRASP65 aa 396-440 / 440.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

SK-Br-3 cell lysates, HepG2, HUVEC, MCF-7, human colon carcinoma tissue, human uterus tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes