DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39] — image 1 of 3

Product details

DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39]

Catalog number ET7107-14

Price for ET7107-14

Size / count 100 µL

About this product

DNA ligase 1 is an enzyme that in humans is encoded by the LIG1 gene. DNA ligase I is the only known eukaryotic DNA ligase involved in both DNA replication and repair, making it the most studied of the ligases. LIG1 encodes DNA ligase I, which functions in DNA replication and the base excision repair process. Eukaryotic DNA ligase 1 catalyzes a reaction that is chemically universal to all ligases. DNA ligase 1 utilizes adenosine triphosphate (ATP) to catalyze the energetically favorable ligation events in both DNA replication and repair. During the synthesis phase (S-phase) of the eukaryotic cell cycle, DNA replication occurs. DNA ligase 1 is responsible for joining Okazaki fragments formed during discontinuous DNA synthesis on the DNA's lagging strand after DNA polymerase δ has replaced the RNA primer nucleotides with DNA nucleotides. If the Okazaki fragments are not properly ligated together, the unligated DNA (containing a ‘nick’) could easily degrade to a double strand break, a phenomenon known to cause genetic mutations.

Specifications

Catalog number: ET7107-14
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: DNA ligase 1 antibody DNA ligase I antibody DNLI1_HUMAN antibody LIG 1 antibody lig1 antibody Ligase I DNA ATP dependent antibody MGC117397 antibody MGC130025 antibody Polydeoxyribonucleotide synthase [ATP] 1 antibody
Abbreviation: LIG1
Swiss Prot: P18858 Human, 37227 Rat
Cellular Locus: Nucleus.
Host: Rabbit
Species Reactivity: Human, Rat
Immunogen: Recombinant protein within Human DNA Ligase I aa 1-131 / 919.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IHC-P
Positive Control: Daudi cell lysate, A431 cell lysate, MCF-7 cell lysate, human tonsil tissue, human spleen tissue, rat testis tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:500 ;IHC-P: 1:50-1:200
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 102 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39]?

The catalog number for DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39] is ET7107-14.

What sizes are available for DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39]?

DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39] is available in the following size(s): 100 µL.

Is DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39] in stock?

DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39] is currently made to order / not held in immediate stock. Contact us for lead time.

What is DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39] used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.