PCK2 Recombinant Rabbit Monoclonal Antibody [JB52-39]

About this product

As a PCK, PCK2 catalyzes the GTP-driven conversion of OAA to PEP as a rate-limiting step in gluconeogenesis. This conversion step serves as a bridge between glycolytic and TCA cycle intermediates in the mitochondria. In pancreatic β-cells, PCK2 regulates glucose-stimulated insulin secretion by recycling GTP generated from the succinyl-CoA synthase reaction. This drives the TCA cycle, converting PEP to pyruvate to acetyl-CoA for the citrate synthase reaction.[9] Since nearly all of the glycolytic reactions upstream of PEP and downstream of glucose-6-phosphate (G6P) are reversible, PCK2-mediated synthesis of PEP could fuel multiple biosynthetic processes, such as serine synthesis, glycerol synthesis, and nucleotide synthesis. Notably, PCK2 preferentially converts OAA derived from lactate and, thus, can promote biosynthesis even under low-glucose conditions. As a result, PCK2 activity contributes to cell growth and survival during stress. While PCK1 is mainly expressed in the liver and kidney, PCK2 is ubiquitously expressed in various cell types, including leukocytes and neurons, as well as in non-gluconeogenic tissues, including pancreas, brain, heart. Moreover, while PCK1 expression is regulated by hormones or nutrients involved in gluconeogenesis, PCK2 is constitutively expressed. These differences indicate that PCK2 may also perform non-gluconeogenic functions.

Specifications

Catalog number

ET7107-29

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

EC 4.1.1.32 antibody GTP mitochondrial precursor antibody HGNC:8725 antibody mitochondrial antibody Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody OTTHUMP00000164700 antibody PCK2 antibody PCKGM_HUMAN antibody PE antibody PEP carboxykinase antibody PEPCK antibody PEPCK deficiency mitochondrial antibody PEPCK M antibody PEPCK-M antibody PEPCK2 antibody Phosphoenolpyruvate carboxykinase [GTP] antibody Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody Phosphoenolpyruvate carboxylase antibody Phosphopyruvate carboxylase antibody

Abbreviation

PEPCK-M, PCK2, PEPCK2

Swiss Prot

Q16822 Human, Q8BH04 Mouse,

Cellular Locus

Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within C-terminal Human PCK2 .

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

Mouse colon tissue lysate, MCF-7 cell lysate, human kidney tissue lysate, HepG2, MCF-7, mouse colon tissue, human kidney tissue, human liver tissue, rat kidney tissue, human colon cancer tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000;IF-Cell: 1:50-1:100;IHC-P: 1:50-1:200;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 71 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes