p53 Recombinant Rabbit Monoclonal Antibody [JB42-26]

About this product

Tumor protein P53, also known as p53, cellular tumor antigen p53 (UniProt name), the Guardian of the Genome, phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation-related protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice). This homolog (originally thought to be, and often spoken of as, a single protein) is crucial in vertebrates, where it prevents cancer formation. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene. The name p53 was given in 1979 describing the apparent molecular mass; SDS-PAGE analysis indicates that it is a 53-kilodalton (kDa) protein. However, the actual mass of the full-length p53 protein (p53α) based on the sum of masses of the amino acid residues is only 43.7 kDa. This difference is due to the high number of proline residues in the protein, which slow its migration on SDS-PAGE, thus making it appear heavier than it actually is. In addition to the full-length protein, the human TP53 gene encodes at least 15 protein isoforms, ranging in size from 3.5 to 43.7 kDa. All these p53 proteins are called the p53 isoforms. The TP53 gene is the most frequently mutated gene (>50%) in human cancer, indicating that the TP53 gene plays a crucial role in preventing cancer formation. TP53 gene encodes proteins that bind to DNA and regulate gene expression to prevent mutations of the genome.

Specifications

Catalog number

ET7107-33

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody

Abbreviation

TP53, P53

Swiss Prot

P04637 Human

Cellular Locus

Cytoplasm. Nucles.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human p53 aa 344-393 / 393.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IP, IF-Cell, IF-Tissue

Positive Control

A431 cell lysate, 293 cell lysate, human stomach carcinoma tissue, human colon carcinoma tissue, Daudi.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:100 ;FC: 1:50-1:100 ;IF-Cell: 1:100-1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes