Cytochrome P450 Reductase Recombinant Rabbit Monoclonal Antibody [JB54-31]

About this product

Cytochrome P450 reductase (EC 1.6.2.4; also known as NADPH:ferrihemoprotein oxidoreductase, NADPH:hemoprotein oxidoreductase, NADPH:P450 oxidoreductase, P450 reductase, POR, CPR, CYPOR) is a membrane-bound enzyme required for electron transfer from NADPH to cytochrome P450 and other heme proteins including heme oxygenase in the endoplasmic reticulum of the eukaryotic cell. Since all microsomal P450 enzymes require POR for catalysis, it is expected that disruption of POR would have devastating consequences. POR knockout mice are embryonic lethal, probably due to lack of electron transport to extrahepatic P450 enzymes since liver-specific knockout of POR yields phenotypically and reproductively normal mice that accumulate hepatic lipids and have remarkably diminished capacity of hepatic drug metabolism. The reduction of cytochrome P450 is not the only physiological function of POR. The final step of heme oxidation by mammalian heme oxygenase requires POR and O2. In yeast, POR affects the ferrireductase activity, probably transferring electrons to the flavocytochrome ferric reductase.

Specifications

Catalog number

ET7107-35

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CPR antibody CYPOR antibody Cytochrome p450 oxidoreductase antibody DKFZp686G04235 antibody FLJ26468 antibody NADPH Cytochrome P450 Reductase antibody NADPH dependent cytochrome P450 reductase antibody NADPH--cytochrome P450 reductase antibody NCPR_HUMAN antibody P450 (cytochrome) oxidoreductase antibody P450 Cytochrome Oxidoreductase antibody P450R antibody por antibody

Abbreviation

NADPH--cytochrome P450 reductase, CPR, P450R, POR, CYPOR

Swiss Prot

P16435 Human, P37040 Mouse, P00388 Rat

Cellular Locus

Endoplasmic reticulum.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human Cytochrome P450 Reductase aa 80-220 / 677.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

A549 cell lysate, mouse lung tissue lysate, HepG2, LOVO, A549, human liver tissue, mouse testis tissue, rat brain tissue, human lung carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 77 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes