PDK2 Recombinant Rabbit Monoclonal Antibody [JB66-93]

About this product

Pyruvate dehydrogenase kinase isoform 2 (PDK2) also known as pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial is an enzyme that in humans is encoded by the PDK2 gene. PDK2 is an isozyme of pyruvate dehydrogenase kinase. The Pyruvate Dehydrogenase (PDH) complex must be tightly regulated due to its central role in general metabolism. Within the complex, there are three serine residues on the E1 component that are sites for phosphorylation; this phosphorylation inactivates the complex. In humans, there have been four isozymes of Pyruvate Dehydrogenase Kinase that have been shown to phosphorylate these three sites: PDK1, PDK2, PDK3, and PDK4. PDK2 has been identified as the most abundant isoform in human tissues. Through many studies, it has been made clear that the activity of this enzyme is essential, even at rest, to regulate glycolysis/carbodydrate oxidation and producing metabolites for oxidative phosphorylation and the electron transport chain. These studies have illustrated that the kinetics of the PDK isoform population, specifically PDK2, is more important in determining PDH activity than measuring PDK activity.

Specifications

Catalog number

ET7107-46

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody mitochondrial antibody PDHK2 antibody PDK2 antibody PDK2_HUMAN antibody Pyruvate dehydrogenase kinase isoform 2 antibody Pyruvate dehydrogenase kinase, isozyme 2 antibody Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody

Abbreviation

PDH kinase 2, PDKII, PDK2, PDHK2

Swiss Prot

Q15119 Human

Cellular Locus

Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human PDK2 aa 358-407 / 407.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IP

Positive Control

Rat heart tissue lysates, rat brain tissue, human colon carcinoma tissue, human fetal skeletal muscle tissue, mouse cerebellum tissue, SH-SY5Y.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes