Creatine kinase B type Recombinant Rabbit Monoclonal Antibody [JB78-34]

About this product

Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein encoded by this gene, CK-BB, consists of a homodimer of two identical brain-type CK-B subunits. BB-CK is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CK-BB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. The encoded protein reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer (CK-BB) in brain and smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone, etc. In heart, a heterodimer (CK-MB) shahil consisting of one CK-B brain-type CK subunit and one CK-M muscle-type CK subunit is prominently expressed. The encoded CK-BB and CK-MB proteins are members of the ATP:guanido phosphotransferase protein family.

Specifications

Catalog number

ET7107-52

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

B CK antibody B-CK antibody BB-CK antibody BCK antibody Brain creatine kinase antibody Ckb antibody CKBB antibody Creatine kinase B antibody Creatine kinase B chain antibody Creatine kinase B type antibody Creatine kinase B-type antibody Creatine Kinase BB Isoenzyme antibody Creatine kinase brain antibody Creatine kinase brain type antibody Creatine phosphokinase BB antibody Epididymis luminal protein 211 antibody Epididymis secretory protein Li 29 antibody HEL 211 antibody HEL S 29 antibody KCRB_HUMAN antibody

Abbreviation

B-CK, CPK-B, CKB, CKBB

Swiss Prot

P12277 Human, Q04447 Mouse, P07335 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human Creatine kinase B type aa 27-118 / 381.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

293 cell lysate, Hela cell lysate, mouse brain tissue lysate, rat brain tissue lysate, 293T, LOVO, rat brain tissue, human prostate tissue, mouse colon tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 43 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes