Proteasome 20S C2 Recombinant Rabbit Monoclonal Antibody [JB89-30]

About this product

Proteasome subunit alpha type-1 is a protein that in humans is encoded by the PSMA1 gene. This protein is one of the 17 essential subunits (alpha subunits 1–7, constitutive beta subunits 1–7, and inducible subunits including beta1i, beta2i, and beta5i) that contributes to the complete assembly of 20S proteasome complex. Crystal structures of isolated 20S proteasome complex demonstrate that the two rings of beta subunits form a proteolytic chamber and maintain all their active sites of proteolysis within the chamber. Concomitantly, the rings of alpha subunits form the entrance of substrate entering the proteolytic chamber. In an inactivated 20S proteasome complex, the gate into the internal proteolytic chamber are guarded by N-terminal tails of specific alpha-subunit. The proteolytic capacity of 20S core particle (CP) can be activated when CP associates with one or two regulatory particles (RP) on one or both side of alpha rings. These regulatory particles include 19S proteasome complexes, 11S proteasome complex, etc. Following the CP-RP association, the confirmation of certain alpha subunits will change and consequently cause the opening of substrate entrance gate.

Specifications

Catalog number

ET7107-59

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

30 kDa prosomal protein antibody HC 2 antibody HC2 antibody Macropain subunit C2 antibody Macropain subunit nu antibody MGC14542 antibody MGC14575 antibody MGC14751 antibody MGC1667 antibody MGC21459 antibody MGC22853 antibody MGC23915 antibody Multicatalytic endopeptidase complex subunit C2 antibody NU antibody PROS 30 antibody PROS-30 antibody PROS30 antibody Proteasome (prosome macropain) subunit alpha type 1 antibody Proteasome alpha 1 subunit antibody Proteasome component C2 antibody Proteasome nu chain antibody Proteasome subunit alpha type 1 antibody Proteasome subunit alpha type I antibody Proteasome subunit alpha type-1 antibody Proteasome subunit nu antibody Protein P30 33K antibody PSA1_HUMAN antibody PSC 2 antibody PSC2 antibody PSMA 1 antibody psmA1 antibody

Abbreviation

PROS-30, PSMA1, HC2, NU, PROS30, PSC2

Swiss Prot

P25786 Human, Q9R1P4 Mouse, P18420 Rat

Cellular Locus

Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Proteasome 20S C2 aa 214-263 / 263.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IP

Positive Control

PC-3M cell lysate, K562 cell lysate, rat testis tissue lysate, mouse testis tissue lysate, mouse colon tissue lysate, human pancreas tissue, rat testis tissue, mouse cerebellum tissue, human liver cancer tissue, K562.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 30 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes