MCM3 Recombinant Rabbit Monoclonal Antibody [JB99-35]

About this product

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation.

Specifications

Catalog number

ET7107-66

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cervical cancer proto oncogene 5 antibody DNA polymerase alpha holoenzyme associated P1 antibody DNA polymerase alpha holoenzyme associated protein P1 antibody DNA polymerase alpha holoenzyme-associated protein P1 antibody DNA replication factor MCM3 antibody DNA replication licensing factor mcm3 antibody HCC 5 antibody HCC5 antibody hRlf beta subunit antibody Human cervical cancer proto oncogene 5 antibody MCM 3 antibody mcm3 antibody MCM3 minichromosome maintenance deficient 3 antibody MCM3_HUMAN antibody MGC1157 antibody Minichromosome maintenance complex component 3 antibody Minichromosome maintenance deficient 3 antibody Minichromosome maintenance protein 3 antibody P1 h antibody P1 MCM3 antibody P1 Protein antibody P1-MCM3 antibody P1.h antibody p102 antibody P102 protein antibody Replication licensing factor beta subunit antibody RLF beta subunit antibody RLF subunit beta antibody RLFB antibody

Abbreviation

MCM3

Swiss Prot

P25205 Human, P25206 Mouse, D3ZFP4 Rat

Cellular Locus

Chromosome, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human MCM3 aa 1-50 / 808.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

Mouse thymus tissue lysate, NIH-3T3 cell lysates, Hela cell lysates, 293T, HepG2, SH-SY-5Y, human tonsil tissue, human placenta tissue, mouse small intestine tissue, K562.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 91 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes