SF2 Recombinant Rabbit Monoclonal Antibody [JB40-76]

About this product

Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns that are required for accurate splice site recognition and the control of alternative splicing. Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites.

Specifications

Catalog number

ET7107-70

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Alternative splicing factor 1 antibody Alternative-splicing factor 1 antibody arginine/serine-rich 1 antibody ASF 1 antibody ASF antibody ASF-1 antibody ASF1 antibody FLJ53078 antibody MGC5228 antibody P33 subunit antibody Pre mRNA splicing factor SF2 P33 subunit antibody pre-mRNA-splicing factor SF2 antibody Serine/arginine-rich splicing factor 1 antibody SF2 antibody SF2P33 antibody SFRS1 antibody Splicing factor 2 alternate splicing factor antibody Splicing factor 2 antibody Splicing factor antibody Splicing factor arginine/serine rich 1 antibody SR Splicing factor 1 antibody SRp30a antibody srsf1 antibody SRSF1_HUMAN antibody

Abbreviation

ASF-1, SRSF1, ASF, SF2, SF2P33, SFRS1, OK/SW-cl.3

Swiss Prot

Q07955 Human, Q6PDM2 Mouse, D4A9L2 Rat

Cellular Locus

Cytoplasm, Nucleus, Spliceosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human SF2 aa 1-50 / 248.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

Mouse heart tissue lysate, mouse liver tissue lysate, K562 cell lysate, A431, human lung carcinoma tissue, human tonsil tissue, human breast tissue, mouse colon tissue, rat epididymis tissue, K562.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000;IF-Cell: 1:50;IHC-P: 1:50-1:200;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 28 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes