MBD3 Recombinant Rabbit Monoclonal Antibody [JB61-47]

About this product

Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5′ cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites, and they include methyl-CpG binding protein-1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues.

Specifications

Catalog number

ET7107-78

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AI181826 antibody AU019209 antibody MBD 3 antibody Mbd3 antibody MBD3: methyl CpG binding domain protein 3 antibody MBD3_HUMAN antibody Methyl CpG binding domain protein 3 antibody Methyl CpG binding protein MBD3 antibody Methyl-CpG-binding domain protein 3 antibody Methyl-CpG-binding protein MBD3 antibody

Abbreviation

MBD3

Swiss Prot

O95983 Human, Q9Z2D8 Mouse, F7EY92 Rat

Cellular Locus

Chromosome, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human MBD3 aa 160-291 / 291.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell, IF-Tissue, IP

Positive Control

MCF-7 cell lysate, A549, NIH/3T3, SH-SY-5Y, rat testis tissue, human placenta tissue, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:500;FC: 1:50-1:100;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 33 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes