PAK2 Recombinant Rabbit Monoclonal Antibody [JB41-84]

About this product

Three recently identified isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase STE20, involved in pheromone signaling. The α, β, and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.

Specifications

Catalog number

ET7107-79

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

C-t-PAK2 antibody CB422 antibody EC 2.7.11.1 antibody Gamma PAK antibody Gamma-PAK antibody hPAK65 antibody Kinase antibody p21 (CDKN1A) activated kinase 2 antibody p21 (CDKN1A)-activated kinase 2a antibody p21 activated kinase 2 antibody p21 protein (Cdc42/Rac)-activated kinase 2 antibody p21 protein Cdc42 Rac activated kinase 2 antibody p21-activated kinase 2 antibody p21-activated kinase, 65-KD antibody p21-activated protein kinase I antibody p21CDKN1A activated kinase 2 antibody p27 antibody p34 antibody p58 antibody p65PAK antibody PAK 2 antibody PAK-2 antibody PAK-2p34 antibody Pak2 antibody PAK2_HUMAN antibody PAK65 antibody PAKgamma antibody S6 H4 kinase antibody S6/H4 kinase antibody Serine threonine protein kinase PAK 2 antibody Serine/threonine protein kinase PAK 2 antibody

Abbreviation

PAK-2, p27, p34, PAK2

Swiss Prot

Q13177 Human, Q8CIN4 Mouse, Q64303 Rat

Cellular Locus

Cytoplasm, Membrane, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within N-terminal Human PAK2 .

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell, IF-Tissue

Positive Control

Mouse thymus tissue lysate, MCF-7 cell lysate, A431, SiHa, human colon cancer tissue, human liver tissue, rat kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:100-1:500 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 58 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes