CD146 Recombinant Rabbit Monoclonal Antibody [JB42-35]

About this product

The tumorigenic and metastatic phenotype of melanoma cells correlates well with an increased expression of cell-cell and cell-matrix adhesion receptors. The human Mel-CAM gene encodes a transmembrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. The deduced human sequence of 603 amino acids consists of a signal peptide, five immunoglobulin-like domains, a transmembrane region and a short cytoplasmic tail. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human mela-nomas and correlates well with development of metastatic disease. Mel-CAM activation initiates an outside-in signaling pathway that involves the protein tyrosine kinases Fyn, FAK and paxillin. Mel-CAM influences the dynamics of Actin cytoskeleton rearrangement and is essential for the maintenance of thymic architecture and function.

Specifications

Catalog number

ET7107-91

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

A32 antigen antibody CD 146 antibody CD146 antibody CD146 antigen antibody Cell surface glycoprotein MUC18 antibody Cell surface glycoprotein P1H12 antibody Gicerin antibody Mcam antibody Melanoma adhesion molecule antibody Melanoma associated antigen A32 antibody Melanoma associated antigen MUC18 antibody Melanoma associated glycoprotein MUC18 antibody Melanoma cell adhesion molecule antibody Melanoma-associated antigen A32 antibody Melanoma-associated antigen MUC18 antibody MelCAM antibody MUC 18 antibody MUC18 antibody MUC18_HUMAN antibody S endo 1 antibody S endo 1 endothelial associated antigen antibody S-endo 1 endothelial-associated antigen antibody

Abbreviation

MCAM, CD146, MUC18

Swiss Prot

P43121 Human, Q8R2Y2 Mouse, Q9EPF2 Rat

Cellular Locus

Membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within C-terminal human CD146 (Cytoplasmic).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell, IF-Tissue

Positive Control

SiHa cell lysates, Hela, HUVEC, rat brain tissue, human tonsil tissue, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000;IF-Cell: 1:100-1:500;IF-Tissue: 1:100-1:500;IHC-P: 1:50-1:200;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 72 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes