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Product details

Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64]

Catalog number ET7108-07

Price for ET7108-07

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Size / count 100 µL

About this product

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.

Specifications

Catalog number
ET7108-07
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
Cancer/testis antigen 106 antibody CDCA 1 antibody CDCA1 antibody Cell division cycle associated 1 antibody Cell division cycle associated protein 1 antibody Cell division cycle-associated protein 1 antibody CT106 antibody hNuf 2 antibody hNuf2 antibody hNuf2R antibody hsNuf2 antibody Kinetochore protein Nuf2 antibody Nuf 2 antibody NUF2 antibody NUF2 NDC80 kinetochore complex component antibody NUF2 NDC80 kinetochore complex component homolog antibody NUF2, S. cerevisiae, homolog of antibody NUF2_HUMAN antibody NUF2R antibody
Abbreviation
hNuf2, hNuf2R, hNuf2R
Swiss Prot
Q9BZD4 Human
Cellular Locus
Centromere, Chromosome, Kinetochore, Nucleus.
Host
Rabbit
Species Reactivity
Human
Immunogen
Recombinant protein within Human Nuf2 aa 1-108 / 464.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, FC
Positive Control
K562 cell lysate, PC-3M cell lysate, THP-1 cell lysate, A431.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000 ;FC: 1:50-1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 54 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64]?

The catalog number for Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64] is ET7108-07.

What sizes are available for Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64]?

Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64] is available in the following size(s): 100 µL.

Is Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64] in stock?

Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64] used for?

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

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