eIF4A3 Recombinant Rabbit Monoclonal Antibody [JG35-33]

About this product

ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation.

Specifications

Catalog number

ET7108-11

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ATP-dependent RNA helicase DDX48 antibody ATP-dependent RNA helicase eIF4A-3 antibody DDX48 antibody DEAD box protein 48 antibody eIF-4A-III antibody eIF4A-III antibody EIF4A3 antibody eIF4AIII antibody Eukaryotic initiation factor 4A-III antibody Eukaryotic initiation factor 4A-like NUK-34 antibody Eukaryotic translation initiation factor 4A isoform 3 antibody hNMP 265 antibody IF4A3_HUMAN antibody NMP 265 antibody NMP265 antibody Nuclear matrix protein 265 antibody NUK34 antibody

Abbreviation

eIF-4A-III, NMP 265, hNMP 265, EIF4A3, DDX48, KIAA0111

Swiss Prot

P38919 Human, Q91VC3 Mouse, Q3B8Q2 Rat

Cellular Locus

Cytoplasm, Nucleus, Spliceosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within N-terminal Human eIF4A3 .

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell, IF-Tissue, IP

Positive Control

SiHa cell lysates, LOVO, MCF-7, SiHa, human tonsil tissue, human colon carcinoma, human prostate cancer tissue, mouse skin tissue, rat skin tissue, human lymph nodes tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 47 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes