TREX1 Recombinant Rabbit Monoclonal Antibody [JG35-71]

About this product

Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Specifications

Catalog number

ET7108-16

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

3' 5' exonuclease TREX1 antibody 3' repair exonuclease 1 antibody AGS1 antibody AGS5 antibody CRV antibody Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody DKFZp434J0310 antibody DNase III antibody DRN3 antibody HERNS antibody Three prime repair exonuclease 1 antibody TREX1 antibody

Abbreviation

DNase III, TREX1

Swiss Prot

Q9NSU2 Human

Cellular Locus

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human TREX1 aa 110-314 / 314.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

A431 cell lysate, SK-Br-3 cell lysate, Hela, LOVO, SiHa, human colon tissue, human prostate carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 33 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes