Emi1 Recombinant Rabbit Monoclonal Antibody [JG35-83] — image 1 of 3

Product details

Emi1 Recombinant Rabbit Monoclonal Antibody [JG35-83]

Catalog number ET7108-20

Price for ET7108-20

Size / count 100 µL

About this product

Emi1 (for early mitotic inhibitor) regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Emi1 is required to arrest unfertilized eggs at metaphase of meiosis II and may be the long-sought mediator of CSF activity. Human Emi1 is similar to Xenopus laevis Emi1, which inhibits the APC (Cdc20) ubiquitination complex to allow accumulation of cyclin B.

Specifications

Catalog number: ET7108-20
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: Early mitotic inhibitor 1 antibody Emi 1 antibody EMI1 antibody F box only protein 5 antibody F box protein 5 antibody F box protein Fbx 5 antibody F box protein Fbx5 antibody F-box only protein 5 antibody FBX5 antibody FBX5_HUMAN antibody Fbxo31 antibody fbxo5 antibody
Abbreviation: FBXO5, EMI1, FBX5
Swiss Prot: Q9UKT4 Human, Q7TSG3 Mouse, B0BNA4 Rat
Cellular Locus: Cytoplasm. Cytoskeleton. Nucleus.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein within Human Emi1 aa 128-244 / 447.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IHC-P
Positive Control: LOVO, Siha, human tonsil tissue, mouse hippocampus tissue, mouse cerebellum tissue, Human kidney, human colon carcinoma.
Concentration: 1 mg/mL
Dilution: IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;WB: 1:500
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 50 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Emi1 Recombinant Rabbit Monoclonal Antibody [JG35-83]?

The catalog number for Emi1 Recombinant Rabbit Monoclonal Antibody [JG35-83] is ET7108-20.

What sizes are available for Emi1 Recombinant Rabbit Monoclonal Antibody [JG35-83]?

Emi1 Recombinant Rabbit Monoclonal Antibody [JG35-83] is available in the following size(s): 100 µL.

Is Emi1 Recombinant Rabbit Monoclonal Antibody [JG35-83] in stock?

Emi1 Recombinant Rabbit Monoclonal Antibody [JG35-83] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Emi1 Recombinant Rabbit Monoclonal Antibody [JG35-83] used for?

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.