RHEB Recombinant Rabbit Monoclonal Antibody [JG37-12]

About this product

H-, K- and N-Ras represent the prototype members of a family of small G proteins which are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound rather than the inactive GDP-bound state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 or 87 mutants of R-Ras (analogous to positions 12 and 61 in H-Ras) have been shown to be capable of activating oncogenic function. Ras p21 in its active GTP binding state binds to Raf-1, resulting in activation of the MAP kinase signaling cascade. An additional member of the Ras family, Rheb (Ras-related GTP-binding protein), also interacts with Raf-1. This interaction is potentiated by growth factors and agents that increase cAMP levels.

Specifications

Catalog number

ET7108-44

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Ras homolog enriched in brain 2, formerly antibody GTP binding protein Rheb antibody GTP-binding protein Rheb antibody MGC111559 antibody Ras homolog enriched in brain 2 antibody Ras homolog enriched in brain antibody RHEB 2 antibody Rheb antibody RHEB_HUMAN antibody RHEB2 antibody RHEB2, formerly antibody

Abbreviation

RHEB, RHEB2

Swiss Prot

Q15382 Human, Q921J2 Mouse

Cellular Locus

Cytoplasm. Golgi apparatus. Endoplasmic reticulum.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within Human RHEB aa 21-108 / 184.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

Wild-type Raw264.7 whole cell lysate, mouse placenta tissue lysates, Neuro-2a, A431, SH-SY5Y, human liver tissue, human colon tissue, human fetal skeletal muscle tissue, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 20 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes