HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66] — image 1 of 3

Product details

HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66]

Catalog number ET7108-50

Price for ET7108-50

Size / count 100 µL

About this product

Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1α. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the β-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1α. This hydroxylation event blocks the association of HIF-1α with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1α. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like β-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24.

Specifications

Catalog number: ET7108-50
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: DKFZp762F1811 antibody Factor inhibiting HIF-1 antibody Factor inhibiting HIF1 antibody FIH 1 antibody FIH-1 antibody FIH1 antibody FLJ20615 antibody FLJ22027 antibody HIF1AN antibody HIF1N_HUMAN antibody Hypoxia inducible factor 1 alpha inhibitor antibody Hypoxia inducible factor 1 alpha subunit inhibitor antibody Hypoxia inducible factor asparagine hydroxylase antibody Hypoxia-inducible factor 1-alpha inhibitor antibody Hypoxia-inducible factor asparagine hydroxylase antibody Peptide aspartate beta dioxygenase antibody
Abbreviation: FIH-1, HIF1AN, FIH1
Swiss Prot: Q9NWT6 Human
Cellular Locus: Nucleus. Cytoplasm.
Host: Rabbit
Species Reactivity: Human
Immunogen: Synthetic peptide within Human HIF1AN aa 1-50 / 349.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC
Positive Control: A549 cell lysate, Jurkat cell lysate, LOVO, SiHa, human prostate carcinoma tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: 40 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66]?

The catalog number for HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66] is ET7108-50.

What sizes are available for HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66]?

HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66] is available in the following size(s): 100 µL.

Is HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66] in stock?

HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66] is currently made to order / not held in immediate stock. Contact us for lead time.

What is HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66] used for?

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.