hUPF1 Recombinant Rabbit Monoclonal Antibody [JG38-44]

About this product

In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability. Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5' to 3' helicase activity. In addition, Rent1 is an RNA-binding protein whose activity is modulated by ATP and directly interacts with Rent2, which is a mammalian homolog of Upf2p. Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by two separate genes. Rent3b (also known as Rent3X) is encoded by a X-linked gene and localizes primarily to the nucleus, while Rent 1 and Rent 2 localize primarily in the cytoplasm. Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm.

Specifications

Catalog number

ET7108-70

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ATP dependent helicase RENT1 antibody ATP-dependent helicase RENT1 antibody Delta helicase antibody FLJ43809 antibody FLJ46894 antibody HUPF 1 antibody hUpf1 antibody KIAA0221 antibody Nonsense mRNA reducing factor 1 antibody NORF 1 antibody NORF1 antibody pNORF 1 antibody pNORF1 antibody Regulator of nonsense transcripts 1 antibody RENT 1 antibody RENT1 antibody RENT1_HUMAN antibody Smg 2 antibody Smg 2 homolog nonsense mediated mRNA decay factor antibody UP Frameshift 1 antibody Up frameshift mutation 1 homolog (S. cerevisiae) antibody Up frameshift mutation 1 homolog antibody Up frameshift suppressor 1 homolog antibody Up-frameshift suppressor 1 homolog antibody UPF 1 antibody UPF 1 regulator of nonsense transcripts homolog antibody upf1 antibody UPF1 regulator of nonsense transcripts homolog antibody UPF1 RNA helicase and ATPase antibody Yeast Upf1p homolog antibody

Abbreviation

NORF1, hUpf1, UPF1, KIAA0221, RENT1

Swiss Prot

Q92900 Human, Q9EPU0 Mouse

Cellular Locus

Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within Human hUPF1 aa 1-120 / 1129.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IP, IHC-P, FC

Positive Control

PC-3M cell lysates, A431, LOVO, PC-3M, human colon tissue, human prostate carcinoma tissue, human kidney tissue, mouse brain tissue, Hela.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100;IP: 1:10-1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

124 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes