IQGAP1 Recombinant Rabbit Monoclonal Antibody [JG38-74]

About this product

IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42.

Specifications

Catalog number

ET7108-79

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

HUMORFA01 antibody IQ motif containing GTPase activating protein 1 antibody IQGA1_HUMAN antibody Iqgap1 antibody KIAA0051 antibody p195 antibody Ras GTPase activating like protein 1 antibody Ras GTPase-activating-like protein IQGAP1 antibody RasGAP-like with IQ motifs antibody SAR1 antibody

Abbreviation

IQGAP1, KIAA0051

Swiss Prot

P46940 Human, Q9JKF1 Mouse

Cellular Locus

Cell membrane. Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within Human IQGAP1 aa 10-100 / 1657.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

HUVEC cell lysate, A431 cell lysate, Mouse placenta tissue lysate, Mouse lung tissue lysate, Rat placenta tissue lysate, Rat lung tissue lysate, A431, Hela, SiHa, human lung tissue, mouse lung tissue, rat lung tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 189 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes