p54nrb/NONO Recombinant Rabbit Monoclonal Antibody [JG87-38]

About this product

Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing.

Specifications

Catalog number

ET7108-81

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

52 kDa subunit antibody 54 kDa nuclear RNA and DNA binding protein antibody 54 kDa nuclear RNA- and DNA-binding protein antibody 55 kDa nuclear protein antibody DNA binding p52/p100 complex 52 kDa subunit antibody DNA-binding p52/p100 complex antibody NMT 55 antibody NMT55 antibody Non Pou domain containing octamer (ATGCAAAT) binding protein antibody Non POU domain containing octamer binding antibody Non POU domain containing octamer binding protein antibody Non-POU domain-containing octamer-binding protein antibody Nono antibody NonO protein antibody NONO_HUMAN antibody NRB 54 antibody NRB antibody NRB54 antibody Nuclear RNA binding protein 54kD antibody P54 antibody p54 (nrb) antibody p54nrb antibody PPP1R114 antibody Protein phosphatase 1 regulatory subunit 114 antibody

Abbreviation

NonO protein, p54nrb, NONO, NRB54

Swiss Prot

Q15233 Human, Q99K48 Mouse, Q5FVM4 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human p54nrb aa 365-471 / 471.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

293, SH-SY-5Y, SiHa, rat kidney tissue, human liver tissue, human colon tissue, human prostate cancer tissue, mouse testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 54 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes