p150 CAF1 Recombinant Rabbit Monoclonal Antibody [JG38-98]

About this product

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated Histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Specifications

Catalog number

ET7108-83

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CAF 1 antibody CAF 1 subunit A antibody CAF antibody CAF I 150 kDa subunit antibody CAF I p150 antibody CAF-1 subunit A antibody CAF-I 150 kDa subunit antibody CAF-I p150 antibody CAF1 antibody CAF1A_HUMAN antibody CAF1B antibody CAF1P150 antibody CHAF1A antibody Chromatin assembly factor 1 subunit A antibody Chromatin Assembly Factor 1 Subunit A p150 antibody Chromatin assembly factor I (150 kDa) antibody Chromatin assembly factor I 150 kDa antibody Chromatin assembly factor I p150 subunit antibody DCAF1 antibody hp150 antibody MGC71229 antibody P150 antibody

Abbreviation

CAF-1 subunit A, CAF-I 150 kDa subunit, CAF-I p150, hp150

Swiss Prot

Q13111 Human

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human p150 CAF1 aa 81-195 / 956.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IP, IHC-P, FC

Positive Control

K562 cell lysates, A431 cell lysates, 293T, A431, LOVO, human tonsil tissue, human colon tissue, Daudi, human testis tissue, human lymph nodes tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;IP: 1:10-1:50;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

107 kDa (Predicted band size)

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes