SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25] — image 1 of 3

Product details

SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25]

Catalog number ET7109-32

Price for ET7109-32

Size / count 100 µL

About this product

SRP54, a component of the signal recognition particle (SRP), recognizes the signal peptide of secretory proteins and interacts with the signal recognition particle receptor to target the ribosome and the associated nascent chain to the endoplasmic reticulum. By functional analysis showed that SRP54 binds to SRP RNA via the M-domain, but only in the presence of RNA-bound SRP19, and that it associates with the signal peptide of nascent polypeptide chains. RNA interaction requires the presence of a loop in the C-terminal M-domain. Signal peptide recognition most likely involves methionine-rich loops. During signal peptide recognition, SRP54 is positioned at the exit site close to ribosomal proteins L23a (602326) and L35. When SRP54 contacts the signal recognition particle receptor (182180), SRP54 is rearranged such that it is no longer close to L23a. This repositioning may allow the translocon to dock with the ribosome, leading to insertion of the signal peptide into the translocation channel.

Specifications

Catalog number: ET7109-32
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: Signal recognition particle 54 kDa protein antibody Signal recognition particle 54kDa antibody SRP 54 antibody SRP54 antibody SRP54_HUMAN antibody
Abbreviation: SRP54, SRP54
Swiss Prot: P61011 Human, P14576 Mouse, Q6AYB5 Rat
Cellular Locus: Cytoplasm, Nucleus.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide within Human SRP54 aa 441-490 / 504.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IHC-P, FC, IP
Positive Control: MCF-7 cell lysate, HepG2 cell lysate, rat brain tissue, human breast carcinoma tissue, human kidney tissue, mouse brain tissue, HepG2.
Concentration: 1 mg/mL
Dilution: WB: 1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: 1:10-1:50
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 56 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25]?

The catalog number for SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25] is ET7109-32.

What sizes are available for SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25]?

SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25] is available in the following size(s): 100 µL.

Is SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25] in stock?

SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25] is currently made to order / not held in immediate stock. Contact us for lead time.

What is SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25] used for?

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.