RPA32/RPA2 Recombinant Rabbit Monoclonal Antibody [JE45-59]

About this product

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Specifications

Catalog number

ET7109-41

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

60S acidic ribosomal protein P1 antibody AA409079 antibody AI325195 antibody AU020965 antibody ik:tdsubc_2g1 antibody M (2)21C antibody MGC137236 antibody OTTHUMP00000004008 antibody p32 antibody p34 antibody RCJMB04_6d17 replication protein A2, 32kDa antibody REPA2 antibody Replication factor A protein 2 antibody Replication protein A 32 kDa subunit antibody Replication protein A 32kDa subunit antibody Replication protein A 34 kDa subunit antibody Replication protein A antibody Replication Protein A2 (32kDa) antibody Replication protein A2 antibody Replication protein A2, 32kDa antibody RF-A protein 2 antibody Rf-A2 antibody RFA antibody RFA2_HUMAN antibody RP-A p32 antibody RP-A p34 antibody RP21C antibody RPA 2 antibody RPA 32 antibody RPA antibody Rpa2 antibody RPA32 antibody RPA34 antibody RpLP1 antibody RpP2 antibody xx:tdsubc_2g1 antibody zgc:109822 antibody

Abbreviation

RP-A p32, RF-A protein 2, RP-A p34, RPA2, REPA2, RPA32, RPA34

Swiss Prot

P15927 Human, Q62193 Mouse, Q63528 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human RPA32 / RPA2 aa 221-270 / 270.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IP, IHC-P

Positive Control

Mouse testis tissue, A431, SH-SY-5Y, SiHa, 293, Hela, HepG2, rat brain tissue, human colon tissue, human breast cancer tissue, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IP: 1:10-1:50 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 29 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes