BOB1 Recombinant Rabbit Monoclonal Antibody [JE47-78]

About this product

POU domain class 2-associating factor 1 is a protein that in humans is encoded by the POU2AF1 gene. Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Reduced activation of Bob1-deficient B cells following antigenic stimulation is often reported. The action of Bob1 on B cell maturation and tolerance could be mediated by effects on B cell activation, in part by regulating the expression of immunosuppressive miRNAs. Bob1 has long been considered a B cell‐specific factor that interacts with the transcription factors Oct1 and Oct2 to enhance octamer‐dependent transcription. Bob1 can directly bind to and transactivate the promoters of Bcl6 and Btla. The expression of BOB1 has proven useful for identifying certain lymphomas as being B-cell lymphomas, as exemplified in studies which use BAB1 expression to help identify lymphomas as being diffuse large B-cell lymphomas, not otherwise specified.

Specifications

Catalog number

ET7109-68

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

B cell Oct binding protein 1 antibody B cell specific coactivator OBF 1 antibody B cell specific coactivator OBF1 antibody B-cell-specific coactivator OBF-1 antibody BOB 1 antibody BOB-1 antibody OBF 1 antibody OBF1 antibody OBF1_HUMAN antibody OCA B antibody OCA-B antibody OCAB antibody OCT binding factor 1 antibody OCT-binding factor 1 antibody POU class 2 associating factor 1 antibody POU domain class 2 associating factor 1 antibody POU domain class 2-associating factor 1 antibody Pou2af1 antibody

Abbreviation

POU2AF1, OBF1

Swiss Prot

Q16633 Human

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within human BOB1 aa 156-256.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

Daudi cell lysate, Raji cell lysate, human B-cell lymphoma tissue, human tonsil tissue, THP-1.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:200-1:1,000 ;FC: 1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 27 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes