CEND1 Recombinant Rabbit Monoclonal Antibody [JE48-13]

About this product

Cullin-associated and neddylation-dissociated (CAND1)/TIP120A is a protein containing multiple HEAT repeats. It functions, in part, as an inhibitor of multiple cullin-RING ubiquitin ligases (CRLs) via binding to cullin-RBX complexes that are both unconjugated to NEDD8 and lack association with substrate recognition subunits (1-3). Indeed, CAND1 has been shown to bind all cullin family members in human cells and analysis of the crystal structure of human CAND1 bound to the CUL1-RBX1 complex suggests that CAND1 inhibits the activity of CRLs by sterically blocking both the substrate recognition subunit binding site and the NEDD8 conjugation site . Conversely, CAND1 binding to cullin-RBX complexes is incompatible with neddylation as NEDD8 conjugated to cullins blocks CAND1 binding, suggesting that CAND1 binds to cullins only after the COP9 signalosome has catalyzed cullin deneddylation. Through its ability to negatively regulate CRL assembly, CAND1 plays an integral part in facilitating CRL activation cycles that allow CRLs to utilize distinct substrate recognition subunits and protects these subunits from undergoing ubiquitin-dependent degradation .

Specifications

Catalog number

ET7109-75

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

BM88 antibody BM88 antigen antibody Cell cycle exit and neuronal differentiation 1 antibody Cell cycle exit and neuronal differentiation protein 1 antibody FLJ90066 antibody MGC34326 antibody

Abbreviation

CEND1, BM88

Swiss Prot

Q8N111 Human, Q9JKC6 Mouse, Q5FVI4 Rat

Cellular Locus

Membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human CEND1 aa 31-140 / 149.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

Rat brain tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, N2A, rat brain tissue, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB:1:1,000-1:5,000;IHC-P: 1:50-1:200;IF-Cell: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes