RSK1 p90 Recombinant Rabbit Monoclonal Antibody [JE48-76]

About this product

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation.

Specifications

Catalog number

ET7109-83

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

90 kDa ribosomal protein S6 kinase 1 antibody dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody dJ590P13.1 antibody EC 2.7.11.1 antibody HU 1 antibody HU1 antibody KS6A1_HUMAN antibody MAP kinase activated protein kinase 1a antibody MAP kinase-activated protein kinase 1a antibody MAPK-activated protein kinase 1a antibody MAPKAP kinase 1a antibody MAPKAPK-1a antibody MAPKAPK1A antibody MGC79981 antibody Mitogen-activated protein kinase-activated protein kinase 1A antibody OTTHUMP00000004113 antibody p90 RSK1 antibody p90-RSK 1 antibody p90rsk antibody p90RSK1 antibody p90S6K antibody pp90RSK1 antibody Ribosomal protein S6 kinase 90kD 1 antibody Ribosomal protein S6 kinase 90kD polypeptide 1 antibody Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody Ribosomal protein S6 kinase alpha 1 antibody Ribosomal protein S6 kinase alpha-1 antibody Ribosomal protein S6 kinase polypeptide 1 antibody Ribosomal S6 kinase 1 antibody RPS6K1 alpha antibody rps6ka antibody Rps6ka1 antibody RSK 1 antibody RSK 1 p90 antibody RSK antibody RSK-1 antibody RSK1 antibody S6K alpha 1 antibody S6K-alpha-1 antibody

Abbreviation

S6K-alpha-1, p90-RSK 1, p90RSK1, p90S6K, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, RSK-1, RPS6KA1, MAPKAPK1A, RSK1

Swiss Prot

Q15418 Human, P18653 Mouse, Q63531 Rat

Cellular Locus

Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human RSK1 p90 aa 1-107 / 735.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

HeLa cell lysate, MCF7 cell lysate, K-562 cell lysate, Daudi cell lysate, PC-12 cell lysate, mouse brain tissue lysate, Hela, human testis tissue, human appendix tissue, human prostate cancer tissue, human small intestine tissue, mouse colon tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50 ;IHC-P: 1:50-1:200 ;FC: 1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 83 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes