ICAD Recombinant Rabbit Monoclonal Antibody [JE49-23]

About this product

DNA fragmentation factor subunit alpha (DFFA), also known as Inhibitor of caspase-activated DNase (ICAD), is a protein that in humans is encoded by the DFFA gene. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. The C-terminal domain of DFFA (DFF-C) consists of four alpha-helices, which are folded in a helix-packing arrangement, with alpha-2 and alpha-3 packing against a long C-terminal helix (alpha-4). The main function of this domain is the inhibition of DFFB by binding to its C-terminal catalytic domain through ionic interactions, thereby inhibiting the fragmentation of DNA in the apoptotic process. In addition to blocking the DNase activity of DFFB, the C-terminal region of DFFA is also important for the DFFB-specific folding chaperone activity, as demonstrated by the ability of DFFA to refold DFFB.

Specifications

Catalog number

ET7109-91

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

A330085O09Rik antibody Caspase activated deoxyribonuclease inhibitor short form antibody DFF 1 antibody DFF 45 antibody DFF alpha antibody DFF-45 antibody DFF1 antibody DFF35 antibody DFF45 antibody DFFA antibody Dffa DNA fragmentation factor, alpha subunit antibody DFFA_HUMAN antibody DNA fragmentation factor 45 kDa subunit antibody DNA Fragmentation Factor Alpha Subunit antibody DNA fragmentation factor subunit alpha antibody DNA fragmentation factor, 45 kD, alpha subunit antibody DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody DNA fragmentation factor, 45kDa, alpha polypeptide antibody DNA fragmentation factor, alpha subunit antibody DNAation factor 45 kDa subunit antibody H13 antibody ICAD antibody ICAD L antibody ICAD S antibody Inhibitor of CAD antibody Inhibitor of Caspase Activated DNase antibody MGC143066 antibody OTTHUMP00000001903 antibody OTTHUMP00000001904 antibody RP23 121D17.3 antibody

Abbreviation

DNA fragmentation factor subunit alpha,DNA fragmentation factor 45 kDa subunit,DFF-45,Inhibitor of CAD,ICAD,DFFA

Swiss Prot

O00273 Human

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within human ICAD aa 37-137.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

Jurkat cell lysate, human stomach tissue lysate, human thyroid tissue, human breast tissue, human small intestine tissue, human pancreas intestine tissue, HCT116.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1000 ;IHC-P: 1:200-1:500 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 37 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes