CD16 Recombinant Rabbit Monoclonal Antibody [JE49-79]

About this product

CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, and macrophages. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16. CD16 is the type III Fcγ receptor. In humans, it exists in two different forms: FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which have 96% sequence similarity in the extracellular immunoglobulin binding regions. While FcγRIIIa is expressed on mast cells, macrophages, and natural killer cells as a transmembrane receptor, FcγRIIIb is only expressed on neutrophils. In addition, FcγRIIIb is the only Fc receptor anchored to the cell membrane by a glycosyl-phosphatidylinositol (GPI) linker, and also plays a significant role in triggering calcium mobilization and neutrophil degranulation. FcγRIIIa and FcγRIIIb together are able to activate degranulation, phagocytosis, and oxidative burst, which allows neutrophils to clear opsonized pathogens.

Specifications

Catalog number

ET7109-97

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CD 16 antibody CD 16a antibody CD16 antibody CD16a antibody CD16a antigen antibody CD16B antibody CD16b antigen antibody Fc fragment of IgG antibody Fc fragment of IgG low affinity IIIa receptor (CD16) antibody Fc fragment of IgG low affinity IIIa receptor antibody Fc fragment of IgG receptor IIIa antibody Fc fragment of IgG, low affinity III, receptor (CD16) antibody Fc fragment of IgG, low affinity III, receptor for (CD16) antibody Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody Fc fragment of IgG, low affinity IIIa, receptor for antibody Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody Fc gamma R3 antibody Fc gamma receptor III 2 (CD 16) antibody Fc gamma receptor III A antibody Fc gamma receptor IIIA antibody Fc gamma receptor IIIb (CD 16) antibody Fc gamma RIII alpha antibody Fc gamma RIII antibody Fc gamma RIII beta antibody Fc gamma RIIIa antibody Fc gamma RIIIb antibody Fc of IgG antibody Fc-gamma receptor III2 (CD 16) antibody Fc-gamma receptor III2 (CD16) antibody Fc-gamma receptor IIIb (CD16) antibody Fc-gamma RIII antibody Fc-gamma RIII-alpha antibody Fc-gamma RIIIa antibody FCG 3 antibody FCG3 antibody FCG3A_HUMAN antibody FCgammaRIIIA antibody FCGR 3 antibody FCGR 3A antibody FCGR3 antibody FCGR3A antibody FCGR3A protein antibody FCGRIII antibody FCGRIII-2 antibody FcR 10 antibody FcR-10 antibody FcR10 antibody FcRIII antibody FcRIIIa antibody IGFR 3 antibody IGFR3 antibody IgG Fc receptor III 1 antibody IgG Fc receptor III 2 antibody IgG Fc receptor III-2 antibody IMD20 antibody immunoglobulin G Fc receptor III antibody immunoglobulin G Fc receptor III-2 antibody Low affinity IIIa receptor antibody Low affinity immunoglobulin gamma Fc region receptor III A antibody Low affinity immunoglobulin gamma Fc region receptor III-A antibody Low affinity immunoglobulin gamma Fc region receptor IIIB antibody neutrophil-specific antigen NA antibody

Abbreviation

CD16a antigen,Low affinity immunoglobulin gamma Fc region receptor III-A,Fc-gamma RIII,Fc-gamma RIII-alpha,Fc-gamma RIIIa,FcRIII, FcRIIIa,CD16a,FcR-10,IgG Fc receptor III-2,FCGR3A

Swiss Prot

P08637 Human, P08508 Mouse, P27645 Rat

Cellular Locus

Cell membrane, Membrane, Secreted.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human CD16 aa 151-254 / 254.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

THP-1 cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, rat spleen tissue lysate, rat thymus tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 29 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes