Phospho-KAP1 (S824) Recombinant Rabbit Monoclonal Antibody [JE50-99]

About this product

TIF1β is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs – an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit. TIF1β is a corepressor for KRAB (Kruppel associated box) domain containing zinc finger proteins. The KRAB domain containing zinc finger proteins are a large group of transcription factors that are vertebrate-specific, varied in their expression patterns between species, and thought to regulate gene transcription programs that control speciation. TIF1β has been shown to be essential for early embryonic development and spermatogenesis. It functions to either activate or repress transcription in response to environmental or developmental signals by chromatin remodeling and histone modification. The recruitment and association of TIF1β with heterochromatin protein (HP1) is essential for transcriptional repression, and for progression through differentiation of F9 embryonic carcinoma cells. TIF1β also plays a role in the DNA damage response. Phosphorylation of TIF1β on Ser842 occurs in an ATM-dependent manner in response to genotoxic stress and is thought to be essential for chromatin relaxation, which is in turn required for the DNA damage response.

Specifications

Catalog number

ET7110-11

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

E3 SUMO protein ligase TRIM28 antibody E3 SUMO-protein ligase TRIM28 antibody FLJ29029 antibody KAP 1 antibody KAP-1 antibody KRAB associated protein 1 antibody KRAB interacting protein 1 antibody KRAB-associated protein 1 antibody KRAB-interacting protein 1 antibody KRIP 1 antibody KRIP-1 antibody KRIP1 antibody Nuclear corepressor KAP 1 antibody Nuclear corepressor KAP-1 antibody RING finger protein 96 antibody RNF96 antibody TF1B antibody TIF1 beta antibody TIF1-beta antibody TIF1B antibody TIF1B_HUMAN antibody Transcription intermediary factor 1 beta antibody Transcription intermediary factor 1-beta antibody Trim28 antibody Tripartite motif containing 28 antibody tripartite motif containing protein 28 antibody Tripartite motif-containing protein 28 antibody

Abbreviation

TRIM28

Swiss Prot

Q13263 Human, Q62318 Mouse, O08629 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser824 of human KAP1.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP, Dot Blot, IF-Cell

Positive Control

HeLa treated with 20μM Etoposide for 2 hours cell lysate, HeLa treated with 100μM Etoposide for 4 hours cell lysate, C6 treated with 25μM Etoposide for 5 hours cell lysate, NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate, HeLa cells treated with 25μM Etoposide for 5 hours, NIH/3T3 cells treated with 25μM Etoposide for 5 hours, human tonsil tissue, human breast tissue, human gastric carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;IP:1:10-1:50 ;Dot Blot: Use at an assay dependent concentration. ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 89 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes