TIMM50 (Tim50) Recombinant Rabbit Monoclonal Antibody [JE51-61]

About this product

Mitochondrial import inner membrane translocase subunit TIM50 is an enzyme that in humans is encoded by the TIMM50 gene. Tim50 is a subunit of the Tim23 translocase complex in the inner mitochondrial membrane. Mutations in TIMM50 can lead to epilepsy, severe intellectual disability, and 3-methylglutaconic aciduria. TIMM50 expression is increased in breast cancer cells and decreased in hypertrophic hearts. The TIMM50 gene is located on the q arm of chromosome 19 in position 13.2 and spans 13,373 base pairs. The gene produces a 39.6 kDa protein composed of 353 amino acids. This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex, which mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. The Tim50 protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This gene in human cells results in the release of cytochrome c and apoptosis. This protein plays a role in maintaining the membrane permeability barrier. The intermembrane space domain of Tim50 induces the translocation pore of the TIM23 channel to close.

Specifications

Catalog number

ET7110-24

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Homolog of yeast Tim50 antibody Import inner membrane translocase subunit TIM 50 antibody Import inner membrane translocase subunit TIM50 antibody Import inner membrane translocase subunit TIM50 mitochondrial antibody MGC102733 antibody Mitochondrial import inner membrane translocase subunit TIM50 antibody TIM 50 antibody TIM 50L antibody TIM50 antibody Tim50 like protein antibody TIM50_HUMAN antibody TIM50L antibody TIMM 50 antibody TIMM50 antibody translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody Translocase of inner mitochondrial membrane 50 homolog antibody Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody

Abbreviation

TIMM50

Swiss Prot

Q3ZCQ8 Human, Q9D880 Mouse

Cellular Locus

Mitochondrion inner membrane, nucleus speckle.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within Human TIM50 aa 1-109 / 353.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

A549 cell lysate, K562 cell lysate, MCF-7 cell lysate, human liver carcinoma tissue, human colon carcinoma tissue, human breast carcinoma tissue, human liver tissue, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 40 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes