DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50] — image 1 of 2

Product details

DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50]

Catalog number ET7110-31

Price for ET7110-31

Size / count 100 µL

About this product

The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

Specifications

Catalog number: ET7110-31
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: Activator of S phase kinase antibody ASK antibody CHIF antibody Chiffon homolog A antibody DBF4 antibody DBF4 homolog (S. cerevisiae) antibody DBF4 homolog antibody DBF4 type zinc finger containing protein 1 antibody DBF4 zinc finger A antibody DBF4 zinc finger antibody DBF4, S. cerevisiae, homolog of antibody DBF4-type zinc finger-containing protein 1 antibody DBF4A antibody DBF4A_HUMAN antibody Dumbbell former 4, S. cerevisiae, homolog of antibody OTTHUMP00000161661 antibody Protein DBF4 homolog A antibody ZDBF1 antibody Zinc finger, DBF-type containing 1 antibody
Abbreviation: DBF4
Swiss Prot: Q9UBU7 Human, Q9QZ41 Mouse
Cellular Locus: Nucleus.
Host: Rabbit
Species Reactivity: Human, Mouse
Immunogen: Recombinant protein within Human DBF4 aa 1-107 / 674.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, FC
Positive Control: 293T cell lysate, Hela cell lysate, SW480 cell lysate, Jurkat cell lysate, F9.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:2,000 ;FC: 1:50-1:100
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 77 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50]?

The catalog number for DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50] is ET7110-31.

What sizes are available for DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50]?

DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50] is available in the following size(s): 100 µL.

Is DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50] in stock?

DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50] is currently made to order / not held in immediate stock. Contact us for lead time.

What is DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50] used for?

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

Is this product classed as hazardous or restricted for shipping?

Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.