eIF3B Recombinant Rabbit Monoclonal Antibody [JE54-00]

About this product

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

Specifications

Catalog number

ET7110-36

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

eIF-3-eta antibody eIF3 eta antibody eIF3 p110 antibody eIF3 p116 antibody EIF3-ETA antibody EIF3-P110 antibody EIF3-P116 antibody eIF3b antibody EIF3B_HUMAN antibody EIF3S9 antibody EIF3S9, formerly antibody Eukaryotic translation initiation factor 3, subunit B antibody Eukaryotic translation initiation factor 3 subunit 9 antibody eukaryotic translation initiation factor 3 subunit 9 eta antibody Eukaryotic translation initiation factor 3 subunit B antibody eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) antibody eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa antibody eukaryotic translation initiation factor 3, subunit 9, formerly antibody hPrt1 antibody Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of antibody PRT1 antibody Prt1 homolog antibody

Abbreviation

EIF3B

Swiss Prot

P55884 Human, Q8JZQ9 Mouse, Q4G061 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human eIF3B aa 81-220 / 814.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

A431 cell lysate, 293T cell lysate, MCF-7, MG-63, human colon tissue, human kidney tissue, mouse testis tissue, rat cerebellum tissue.

Concentration

1 mg/mL

Dilution

WB:1:500-1:2,000;IF-Cell:1:50-1:200;IHC-P:1:50-1:200;FC:1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 92 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes