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Product details

ARF5 Recombinant Rabbit Monoclonal Antibody [JE53-83]

Catalog number ET7110-46

Price for ET7110-46

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Size / count 100 µL

About this product

The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins, and activation of phospho-lipase D. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization.

Specifications

Catalog number
ET7110-46
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
ADP ribosylation factor 5 antibody ADP-ribosylation factor 5 antibody ARF 5 antibody Arf5 antibody ARF5_HUMAN antibody
Abbreviation
ARF5
Swiss Prot
P84085 Human, P84084 Mouse
Cellular Locus
Golgi apparatus, trans-Golgi network membrane, perinuclear region, membrane.
Host
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant protein within Human ARF5 aa 60-180 / 180.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P
Positive Control
HepG2 cell lysates, human liver tissue, human breast carcinoma tissue, mouse lung tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500 ;IHC-P: 1:50-1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 21 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for ARF5 Recombinant Rabbit Monoclonal Antibody [JE53-83]?

The catalog number for ARF5 Recombinant Rabbit Monoclonal Antibody [JE53-83] is ET7110-46.

What sizes are available for ARF5 Recombinant Rabbit Monoclonal Antibody [JE53-83]?

ARF5 Recombinant Rabbit Monoclonal Antibody [JE53-83] is available in the following size(s): 100 µL.

Is ARF5 Recombinant Rabbit Monoclonal Antibody [JE53-83] in stock?

ARF5 Recombinant Rabbit Monoclonal Antibody [JE53-83] is currently made to order / not held in immediate stock. Contact us for lead time.

What is ARF5 Recombinant Rabbit Monoclonal Antibody [JE53-83] used for?

The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

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