Estrogen Receptor alpha Recombinant Rabbit Monoclonal Antibody [JE26-14]

About this product

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades.

Specifications

Catalog number

ET7110-60

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

7*/654 isoform antibody 7*/819 2 isoform antibody 7*/822 isoform antibody 8*/901 isoform antibody 8*/941 isoform antibody DKFZp686N23123 antibody ER alpha antibody ER antibody ER-alpha antibody Era antibody ESR antibody ESR1 antibody ESR1_HUMAN antibody ESRA antibody Estradiol receptor antibody Estrogen nuclear receptor alpha antibody Estrogen receptor 1 antibody Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody Estrogen receptor alpha antibody Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody Estrogen receptor alpha delta 4 +49 isoform antibody Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody Estrogen receptor alpha E1 E2 1 2 antibody Estrogen receptor alpha E1 N2 E2 1 2 antibody Estrogen receptor antibody ESTRR antibody NR3A1 antibody Nuclear receptor subfamily 3 group A member 1 antibody

Abbreviation

ESR1

Swiss Prot

P03372 Human, P19785 Mouse

Cellular Locus

Cell membrane, nucleus, cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human Estrogen Receptor alpha aa 1-50 / 595.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

MCF-7 cell lysates, human endometrium tissue, mouse endometrium tissue.

Concentration

1 mg/mL

Dilution

WB:1:500-1:2,000 ;IHC-P: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 66 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes