XPD Recombinant Rabbit Monoclonal Antibody [JE55-02]

About this product

ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.

Specifications

Catalog number

ET7110-93

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

TFIIH 80 kDa subunit antibody Basic transcription factor 2 80 kDa subunit antibody BTF2 p80 antibody COFS 2 antibody COFS2 antibody CXPD antibody DNA excision repair protein ERCC 2 antibody DNA excision repair protein ERCC-2 antibody DNA repair protein complementing XP D cells antibody DNA repair protein complementing XP-D cells antibody EM9 antibody ERCC 2 antibody ERCC2 antibody ERCC2_HUMAN antibody Excision repair 2 antibody Excision repair cross complementing rodent repair deficiency complementation antibody Excision repair cross complementing rodent repair deficiency, complementation group 2 antibody MAG antibody MGC102762 antibody MGC126218 antibody MGC126219 antibody OTTHUMP00000045860 antibody OTTHUMP00000045861 antibody OTTHUMP00000045862 antibody OTTHUMP00000045863 antibody TFIIH 80 kDa subunit antibody TFIIH basal transcription factor complex 80 kDa subunit antibody TFIIH Basal Transcription Factor Complex Helicase Subunit antibody TFIIH basal transcription factor complex helicase XPD subunit antibody TFIIH basal transcription factor complex p80 subunit antibody TFIIH p80 antibody TTD antibody Xeroderma pigmentosum complementary group D antibody Xeroderma pigmentosum group D complementing protein antibody Xeroderma pigmentosum group D-complementing protein antibody XPD antibody XPDC antibody

Abbreviation

ERCC2

Swiss Prot

P18074 Human

Cellular Locus

Nucleus, spindle.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human XPD aa 620-760 / 760.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

K562 cell lysates, human colon carcinoma tissue, human skin tissue, human smooth muscle tissue, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB:1:500-1:1,000 ;IHC-P:1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 87 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes