Glycerol Kinase Recombinant Rabbit Monoclonal Antibody [JE55-12]

About this product

Glycerol kinase, encoded by the gene GK, is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. Glycerol kinase catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol 3-phosphate. Adipocytes lack glycerol kinase so they cannot metabolize the glycerol produced during triacyl glycerol degradation. This glycerol is instead shuttled to the liver via the blood where it is: Phosphorylated by glycerol kinase to glycerol 3-phosphate. Converted from glycerol 3-phosphate to dihydroxyacetone phosphate (DHAP) via glycerol 3-phosphate dehydrogenase. DHAP can participate in glycolysis or gluconeogenesis. This protein may use the morpheein model of allosteric regulation. Glycerol Kinase (alternative name, ATP:glycerol 3-phosphotransferase or Glycerokinase) adopts a ribonuclease H-like fold consisting of an alpha-beta 2-layer sandwich of CATH family 3.30.420.40. As of March 2010, there were 20 structures of this protein in the PDB, most of which are homodimeric.

Specifications

Catalog number

ET7110-96

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ATP glycerol 3 phosphotransferase antibody ATP:glycerol 3 phosphotransferase antibody ATP:glycerol 3-phosphotransferase antibody D930012N15Rik antibody GK antibody GK1 antibody GKD antibody GLPK_HUMAN antibody Glycerokinase antibody Glycerol kinase antibody OTTHUMP00000023108 antibody OTTHUMP00000023109 antibody OTTHUMP00000215321 antibody

Abbreviation

GK

Swiss Prot

P32189 Human, Q64516 Mouse, Q63060 Rat

Cellular Locus

Mitochondrion outer membrane, cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human Glycerol kinase aa 440-559 / 559.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

Mouse liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, mouse bone marrow tissue lysate, rat testis tissue lysate, rat testis tissue, human liver tissue, human kidney tissue, mouse testis tissue, 293.

Concentration

1 mg/mL

Dilution

WB:1:500-1:2,000 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size 61/57 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes