SUPT5H Recombinant Rabbit Monoclonal Antibody [JE55-25]

About this product

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Specifications

Catalog number

ET7111-01

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DRB sensitivity inducing factor large subunit antibody DRB sensitivity-inducing factor 160 kDa subunit antibody DRB sensitivity-inducing factor large subunit antibody DSIF large subunit antibody DSIF p160 antibody FLJ34157 antibody hSPT 5 antibody hSPT5 antibody SPT 5 antibody SPT 5H antibody SPT5 antibody SPT5H antibody SPT5H_HUMAN antibody Suppressor of Ty 5 homolog antibody Suppressor of Ty 5 homolog (S. cerevisiae) antibody SUPT 5H antibody supt5h antibody Tat cotransactivator 1 protein antibody Tat CT1 antibody Tat CT1 protein antibody Tat-cotransactivator 1 protein antibody Tat-CT1 protein antibody Transcription elongation factor SPT 5 antibody Transcription elongation factor spt5 antibody Transcription factor Tat CT1 antibody

Abbreviation

SUPT5H

Swiss Prot

O00267 Human, O55201 Mouse

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human SPT5 aa 730-870 / 1,087.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

Human placenta tissue lysate, rat skin tissue lysate, F9 cell lysate, A549, MCF-7, rat large intestine tissue, human cervix tissue, human thyroid tissue tissue, human colon carcinoma tissue, human skin tissue, human prostate carcinoma tissue, human breast carcinoma tissue, human stomach carcinoma tissue, human small intestine tissue, human pancreas tissue, mouse colon tissue, mouse heart tissue.

Concentration

1 mg/mL

Dilution

WB:1:500-1:2,000;IF-Cell:1:50-1:100;IHC-P:1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 121 kDa.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes