GTF2F2 Recombinant Rabbit Monoclonal Antibody [JE56-15]

About this product

TFIIF RAP 30 Antibody is a high quality monoclonal TFIIF RAP 30 antibody (also designated TFIIF RAP 30 antibody) suitable for the detection of the TFIIF RAP 30 protein of mouse, rat and human origin. TFIIF RAP 30 Antibody is available as both the non-conjugated anti-TFIIF RAP 30 antibody form, as well as multiple conjugated forms of anti-TFIIF RAP 30 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.

Specifications

Catalog number

ET7111-08

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ATP dependent helicase GTF2F2 antibody ATP-dependent helicase GTF2F2 antibody BTF4 antibody General transcription factor IIF 30 kDa subunit antibody General transcription factor IIF polypeptide 2 antibody General transcription factor IIF subunit 2 antibody General transcription factor IIF, polypeptide 2, 30kDa antibody Gtf2f2 antibody RAP30 antibody T2FB_HUMAN antibody TF2F2 antibody TFIIF antibody TFIIF beta antibody TFIIF-beta antibody Transcription initiation factor IIF subunit beta antibody Transcription initiation factor RAP30 antibody

Abbreviation

GTF2F2

Swiss Prot

P13984 Human, Q01750 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Rat

Immunogen

Synthetic peptide within C-terminal Human GTF2F2.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

Rat testis tissue lysate, A549 cell lysate, HepG2 cell lysate, HL-60 cell lysate, THP-1 cell lysate, Daudi cell lysate, rat testis tissue, human skin tissue, human placenta tissue, human uterus tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

28 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes