HLA-DRB4 Recombinant Rabbit Monoclonal Antibody [JE55-85]

About this product

Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC II α/β dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (β-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.

Specifications

Catalog number

ET7111-17

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DR 4 antibody DR beta 4 chain antibody DR4 antibody DRB1 transplantation antigen antibody DRB4 antibody DRB4_HUMAN antibody HLA class II histocompatibility antigen antibody HLA class II histocompatibility antigen DR beta 4 chain antibody HLA-DRB4 antibody Human leucocyte antigen DRB4 antibody Leukocyte antigen antibody Major histocompatibility complex class II DR beta 4 antibody MHC class II antigen DRB4 antibody MHC class II antigen HLA DR beta antibody MHC class2 antigen antibody MHC HLA DR-beta chain antibody

Abbreviation

HLA-DRB4

Swiss Prot

P13762 Human

Cellular Locus

Lysosome membrane, Endosome membrane, Late endosome membrane, trans-Golgi network membrane, Endoplasmic reticulum membrane, Cell membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide corresponding to the extracellular domain of Human HLA-DRB4.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

Raji cell lysates, human tonsil tissue, human spleen tissue, human breast carcinoma tissue, Jurkat.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

30 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes