PIM2 Recombinant Rabbit Monoclonal Antibody [JE55-57]

About this product

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X (L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.

Specifications

Catalog number

ET7111-29

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

OTTHUMP00000025825 antibody Pim 2 oncogene antibody Pim 2h antibody Pim-2h antibody PIM2 antibody PIM2 Oncogene antibody PIM2_HUMAN antibody Pim2h antibody Proto Oncogene PIM2 antibody Proto-oncogene Pim-2 (serine threonine kinase) antibody Serine/threonine kinase PIM2 antibody Serine/threonine-protein kinase Pim-2 antibody

Abbreviation

PIM2

Swiss Prot

Q9P1W9 Human, Q62070 Mouse

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within C-terminal Human PIM2.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

Rat liver tissue lysate, mouse liver tissue lysate, human small intestine tissue lysate, 293T, A431, rat testis tissue, human liver tissue, human liver carcinoma tissue, human colon carcinoma tissue, mouse small intestine tissue, HepG2.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000;IF-Cell: 1:50-1:100;IHC-P: 1:50-1:200;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

34 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes