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Product details

Histone H3 (acetyl K27) Rabbit Polyclonal Antibody

Catalog number HA500046

Price for HA500046

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Size / count 100 µL

About this product

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.

Specifications

Catalog number
HA500046
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Product Name Alternative
H3 histone family member E pseudogene antibody H3 histone family, member A antibody H3/A antibody H31_HUMAN antibody H3F3 antibody H3FA antibody Hist1h3a antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody histone 1, H3a antibody Histone cluster 1, H3a antibody Histone H3 3 pseudogene antibody Histone H3.1 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody H3K27ac antibody
Abbreviation
Histone H3 H3K27ac
Swiss Prot
P68431 Human, P84243 Human, Q16695 Human, Q71DI3 Human, Q6NXT2 Human
Cellular Locus
Chromosome, Nucleosome core, Nucleus.
Host
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Synthetic peptide within human Histone H3 (acetyl K27) aa 1-50.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IHC-P, ChIP
Positive Control
Hela treated with TSA whole cell lysate, NIH/3T3 treated with TSA whole cell lysate, Hela cell lysate treated with Sodium Butyrate 0.5mM for 24h, human skin tissue, mouse colon tissue, human colon tissue, human colon carcinoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No

Frequently Asked Questions

What is the catalog number for Histone H3 (acetyl K27) Rabbit Polyclonal Antibody?

The catalog number for Histone H3 (acetyl K27) Rabbit Polyclonal Antibody is HA500046.

What sizes are available for Histone H3 (acetyl K27) Rabbit Polyclonal Antibody?

Histone H3 (acetyl K27) Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is Histone H3 (acetyl K27) Rabbit Polyclonal Antibody in stock?

Histone H3 (acetyl K27) Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is Histone H3 (acetyl K27) Rabbit Polyclonal Antibody used for?

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form t

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

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