Histone H3 (acetyl K18) Rabbit Polyclonal Antibody

About this product

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.

Specifications

Catalog number

HA500047

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

H3 histone family member E pseudogene antibody H3 histone family, member A antibody H3/A antibody H31_HUMAN antibody H3F3 antibody H3FA antibody Hist1h3a antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody histone 1, H3a antibody Histone cluster 1, H3a antibody Histone H3 3 pseudogene antibody Histone H3.1 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody H3K18ac antibody

Abbreviation

Histone H3 H3K18ac

Swiss Prot

P68431 Human, P84243 Human, Q16695 Human, Q71DI3 Human, Q6NXT2 Human

Cellular Locus

Chromosome, Nucleosome core, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within human Histone H3 (acetyl K18) aa 1-50.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

Hela cell lysate , untreated, Hela cell lysate, treated with Sodium Butyrate 0.5mM for 24h, Hela cell lysate, treated with trichostatin at 500 ng/ml for 4h, mouse colon, HeLa cells treated with 500ng/mL Trichostatin A for 4 hours.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:100-1:500 ;IF-Cell: 1:500

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No